کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4361847 | 1301426 | 2007 | 12 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Invasive and Adherent Bacterial Pathogens Co-Opt Host Clathrin for Infection Invasive and Adherent Bacterial Pathogens Co-Opt Host Clathrin for Infection](/preview/png/4361847.png)
SummaryInfection by the bacterium Listeria monocytogenes depends on host cell clathrin. To determine whether this requirement is widespread, we analyzed infection models using diverse bacteria. We demonstrated that bacteria that enter cells following binding to cellular receptors (termed “zippering” bacteria) invade in a clathrin-dependent manner. In contrast, bacteria that inject effector proteins into host cells in order to gain entry (termed “triggering” bacteria) invade in a clathrin-independent manner. Strikingly, enteropathogenic Escherichia coli (EPEC) required clathrin to form actin-rich pedestals in host cells beneath adhering bacteria, even though this pathogen remains extracellular. Furthermore, clathrin accumulation preceded the actin rearrangements necessary for Listeria entry. These data provide evidence for a clathrin-based entry pathway allowing internalization of large objects (bacteria and ligand-coated beads) and used by “zippering” bacteria as part of a general mechanism to invade host mammalian cells. We also revealed a nonendocytic role for clathrin required for extracellular EPEC infections.
Journal: - Volume 2, Issue 5, 15 November 2007, Pages 340–351