کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4371814 1302542 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Trypanosoma cruzi epimastigotes: Regulation of myo-inositol transport by effectors of protein kinases A and C
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
پیش نمایش صفحه اول مقاله
Trypanosoma cruzi epimastigotes: Regulation of myo-inositol transport by effectors of protein kinases A and C
چکیده انگلیسی

Inositol is the precursor for most Trypanosoma cruzi surface molecules, including phosphoinositides, glycosylinositolphospholipids and glycosylphosphatidylinositol anchors. As the parasite is an inositol auxotroph, the inositol transport system might be a potential target for new trypanocide drugs, as some of its properties are different from its mammalian counterpart. Here, we investigated the modulation exerted by effectors of PKA and PKC on this transport system to comply with the parasite physiology. Pre-incubation of the cells with either dibutyryl-cyclic AMP (25 μM) or forskolin (30 μM) decreased the myo-inositol uptake by half, this effect being reversed by KT5720 (PKA inhibitor). Conversely, pre-incubation of the cells with PMA (2.8 μg/ml) or serum (5%) had a ≈50% stimulation in myo-inositol uptake, being this effect reversed by staurosporine (0.5 μM) or sphingosine (10 μM). These results allow us to conclude that the myo-inositol transport system in T. cruzi epimastigotes is inhibited by PKA and stimulated by PKC effectors.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 117, Issue 2, October 2007, Pages 171–177
نویسندگان
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