کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4371830 | 1302543 | 2008 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Trypanocidal and leishmanicidal activities of different antimicrobial peptides (AMPs) isolated from aquatic animals
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کلمات کلیدی
RBCTachyplesinMytilinLeishmania braziliensisALFPenaeidinMAGMICAMPLD50FBSRed blood cells - سلولهای قرمز خون3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide - 3- (4،5-Dimethylthiazol-2-yl) -2،5-diphenyltetrazoliumbromideMTT - MTTTrypanosoma cruzi - تریپانوزوم کروزیMinimum inhibitory concentration - حداقل غلظت مهاریPEN - خودکارfetal bovine serum - سرم جنین گاوAnti-lipopolysaccharide factor - فاکتور Anti-lipopolysaccharideAntiparasitic activity - فعالیت ضد انگلMagainin - مگنینAntimicrobial peptide - پپتیدهای ضدمیکروبی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
انگل شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Trypanocidal and leishmanicidal activities of different antimicrobial peptides (AMPs) isolated from aquatic animals Trypanocidal and leishmanicidal activities of different antimicrobial peptides (AMPs) isolated from aquatic animals](/preview/png/4371830.png)
چکیده انگلیسی
Most of the available animal antimicrobial peptides (AMPs) have been tested against bacteria and fungi, but very few against protozoan parasites. In the present study, we investigated the antiparasitic activity of different AMPs isolated from aquatic animals: tachyplesin (Tach, from Tachypleus tridentatus), magainin (Mag, from Xenopus laevis), clavanin (Clav, from Styela clava), penaeidin (Pen, from Litopenaeus vannamei), mytilin (Myt, from Mytilus edulis) and anti-lipopolysaccharide factor (ALF, from Penaeus monodon). The antiparasitic activity was evaluated against the promastigote form of Leishmania braziliensis and epi and trypomastigote forms of Trypanosoma cruzi, through the MTT method. Tach was the most potent peptide, killing completely L. braziliensis and trypomastigote T. cruzi from 12.5 μM, whereas Pen and Clav were weakly active against trypomastigotes and Myt against L. braziliensis, only at a high concentration (100 μM). Tach and Mag were markedly hemolytic at high concentrations, whereas the other peptides caused only a slight hemolysis (<10% up to 50 μM). Our results point to Tach as the only potential candidate for further investigation and potential application as a therapeutic agent.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 118, Issue 2, February 2008, Pages 197-202
Journal: Experimental Parasitology - Volume 118, Issue 2, February 2008, Pages 197-202
نویسندگان
S.E. Löfgren, L.C. Miletti, M. Steindel, E. Bachère, M.A. Barracco,