کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4372180 1302574 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Trypanosoma cruzi: Activity of essential oils from Achillea millefolium L., Syzygium aromaticum L. and Ocimum basilicum L. on epimastigotes and trypomastigotes
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
پیش نمایش صفحه اول مقاله
Trypanosoma cruzi: Activity of essential oils from Achillea millefolium L., Syzygium aromaticum L. and Ocimum basilicum L. on epimastigotes and trypomastigotes
چکیده انگلیسی

Trypanocidal activity of clove (Syzygium aromaticum L.), basil (Ocimum basilicum L.) and yarrow (Achillea millefolium L.) essential oils and some of their constituents (eugenol and linalool) was investigated on Trypanosoma cruzi epimastigote and bloodstream trypomastigote forms. Steam distillation was used to isolate the essential oils, with chemical analyses performed by gas chromatography (GC) and GC coupled to mass spectrometry (GC–MS). The IC50 (concentration that inhibits 50% parasite growth) of the oils and constituents upon T. cruzi was determined by cell counting in a Neubauer chamber. Cell morphology alterations were observed by scanning and transmission electron microscopy. Treatment with oils and constituents demonstrated that they inhibit parasite growth, with clove essential being the most effective one (IC50 = 99.5 μg/ml for epimastigotes and 57.5 μg/ml for trypomastigotes). Ultrastructural alterations were observed mainly in the nucleus.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 116, Issue 3, July 2007, Pages 283–290
نویسندگان
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