کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4372272 | 1302602 | 2006 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification and biochemical characterization of Leishmania strains isolated in Peru, Mexico, and Spain
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کلمات کلیدی
NaClPNAMDHGPiWGAIDHPGM1H NMR - 1H-NMRDNA - DNA یا اسید دزوکسی ریبونوکلئیکEDTA - اتیلن دی آمین تترا استیک اسید Ethylenediaminetetraacetic acid - اتیلینیدامین تتراستیک اسیدdeoxyribonucleic acid - اسید deoxyribonucleicminimal essential medium - حداقل وسایل ضروریSOD - سدSodium chloride - سدیم کلریدproton nuclear magnetic resonance spectroscopy - طیف سنجی رزونانس مغناطیسی هسته ای پروتونIn vitro culture - فرهنگ in vitroMEM - مامانLeishmania species - گونه لیشمانیا
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
انگل شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Identification and biochemical characterization of Leishmania strains isolated in Peru, Mexico, and Spain Identification and biochemical characterization of Leishmania strains isolated in Peru, Mexico, and Spain](/preview/png/4372272.png)
چکیده انگلیسی
Eight Leishmania promastigotes were isolated from different geographical areas: three (LP1, LP2, and LP3) from the provincial department La Libertad and the fourth (LP4) from the department of Cajamarca (northern Peru); another three (LM1, LM2, and LM3) in the province of Campeche (Mexico); and the last (LS1) from a clinical case of a dog in Madrid (Spain). The isolates were characterized by carbohydrate cell-surface residues using agglutinations with four purified lectins, by isoenzyme analysis using different isoenzymes, by analysis of kinetoplast DNA (kDNA) restriction fragment length polymorphism using four different restriction endonucleases and by the final metabolite patterns after in vitro culture. These isolates were compared with four reference strains and typified as: Leishmania (Leishmania) donovani, two strains of L. (L.) infantum, and one species of L. (Viania) peruviana. According to our results and the statistical study, the Peruvian isolates represent three different strains: one would be L. (V.) peruviana, another the strain isolated in Cajamarca (LP4) and the third would include the three strains from the department of La Libertad (LP1, LP2, and LP3), these latter three isolates being phylogenetically closer to the reference strain L. (L.) donovani. Meanwhile, the three isolates from Mexico form a group with close phylogenetic relationships to each other. The isolate from Spain belongs to the species L. (L.) infantum. Thus, a close correlation was drawn between the identity of each strain and its geographical origin.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 112, Issue 1, January 2006, Pages 44-51
Journal: Experimental Parasitology - Volume 112, Issue 1, January 2006, Pages 44-51
نویسندگان
Isabel RodrÃguez-González, Clotilde MarÃn, Franklin Vargas, Ofelia Córdova, Mario Barrera, Ramón Gutiérrez-Sánchez, Jose MarÃa Alunda, Manuel Sánchez-Moreno,