Algae mediate submerged macrophyte response to nutrient and dissolved inorganic carbon loading: A mesocosm study on different species

• Algea-mediated macrophytes responses to nutrients and DIC enrichments were studied.
• Increase nutrients resulted in algea growth and increased light attenuation.
• DIC enrichment resulted in a heavily algea state when combined with nutrients.
• The changes in algal and water chemistry are related to performances of plants.
• Species-specific performances occured when plants in high abundance of algea.

Nutrient and dissolved inorganic carbon are two important factors that influence the development and species composition of submerged macrophyte communities in shallow lake ecosystems. Yet little is known about their potential interactive effects on the submerged macrophytes and competition outcome of macrophyte–phytoplankton. We performed a mesocosm experiment to investigate the growth and photosynthetic performance of three submerged macrophytes in relation to phytoplankton/epiphyton with nutrient and bicarbonate enrichment. During the experimental period (42 d), increase in nutrient loading in water column resulted in a substantial burst of phytoplankton and epiphyton growth and increased light attenuation. When combined with nutrient loading, bicarbonate enrichment also resulted in a heavily phytoplankton- and epiphyton-dominated state, although bicarbonate enrichment per se does not cause the phytoplankton and epiphyton growth. However, increase in nutrient loading in water column had significant negative impact on individual performances (growth and photosynthesis) of the three submerged macrophytes and bicarbonate enrichment increased the effect of eutrophication on two dissected-leaf macrophytes (M. spicatum and E. nuttallii). Furthermore, our results also suggest that species-specific photosynthetic performances occurred when submerged macrophytes in an environment with high abundance of phytoplankton/epiphyton. This study highlighted that increase in nutrient loading and bicarbonate in water column is likely to interactively impact both abiotic and biotic properties of a freshwater ecosystem. The interactions of these two factors could select macrophyte species that are able to resist the shading from phytoplankton/epiphyton, making these species more dominant in natural freshwater ecosystems.