Long-term efficiency of soil stabilization with apatite and Slovakite: The impact of two earthworm species (Lumbricus terrestris and Dendrobaena veneta) on lead bioaccessibility and soil functioning

Remediation soil is exposed to various environmental factors over time that can affect the final success of the operation. In the present study, we assessed Pb bioaccessibility and microbial activity in industrially polluted soil (Arnoldstein, Austria) stabilized with 5% (w/w) of Slovakite and 5% (w/w) of apatite soil after exposure to two earthworm species, Lumbricus terrestris and Dendrobaena veneta, used as model environmental biotic soil factors. Stabilization resulted in reduced Pb bioaccessibility, as assessed with one-step extraction tests and six-step sequential extraction, and improved soil functioning, mirrored in reduced β-glucosidase activity in soil. Both earthworm species increased Pb bioaccessibility, thus decreasing the initial stabilization efficacy and indicating the importance of considering the long-term fate of remediated soil. The earthworm species had different effects on soil enzyme activity, which can be attributed to species-specific microbial populations in earthworm gut acting on the ingested soil.

► Earthworms affect the stabilization efficiency of Slovakite and apatite amendments.
► Pb bioaccessibility in soil may increase when earthworms are present.
► Results of different soil extraction tests can be conflicting.
► The biological functioning of remediated soils was affected by earthworms.
► The two species had different impacts on soil chemical and biological properties.