کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4409971 | 1307520 | 2012 | 6 صفحه PDF | دانلود رایگان |
The effectiveness of genetic bioaugmentation relies on efficient plasmid transfer between donor and recipient cells as well as the plasmid’s phenotype in the recipient cell. In the present study, the effects of varying organic carbon substrates, initial recipient-to-donor cell density ratios, and mixtures of known recipient bacterial strains on the conjugation and function of a TOL plasmid were tested in sterile soil slurry batch reactors. The presence of soil organic carbon was sufficient in ensuring TOL plasmid transconjugant occurrence (up to 2.1 ± 0.5%) for most recipient strains in soil slurry batch mating experiments. The addition of glucose had limited effects on transconjugant occurrence; however, glucose amendment increased the specific toluene degradation rates of some Enterobacteriaceae transconjugants in soil slurry. Initial cell density ratios and mixtures of recipient strains had smaller impacts on plasmid conjugation and resulting phenotype functionality. These observations suggest that genetic bioaugmentation may be improved by minimal altering of environmental conditions.
► Genetic bioaugmentation can be improved by controlling external parameters.
► Transconjugant occurrence and contaminant degradation are key outcomes.
► Soil organic carbon availability affects conjugal transfer of the TOL plasmid.
► Glucose enhances phenotype of Enterobacteriaceae transconjugants in soil slurry.
Journal: Chemosphere - Volume 89, Issue 2, September 2012, Pages 158–163