کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4410388 | 1307541 | 2012 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Stereoselective dissipation of epoxiconazole in grape (Vitis viniferacv. Kyoho) and soil under field conditions Stereoselective dissipation of epoxiconazole in grape (Vitis viniferacv. Kyoho) and soil under field conditions](/preview/png/4410388.png)
Stereoselective dissipation of epoxiconazole had been studied in grape and soil during plant growing under field conditions in this paper. A sensitive and rapid chiral method was developed and validated for the determination of epoxiconazole stereoisomers in grape and soil based on liquid chromatography coupled with triple quadrupole mass spectrometry (LC–MS/MS). Phenomenex Lux Cellulose-1 column was used for enantioseparation with a mixture of acetonitrile/water (90/10, v/v) as mobile phase at flow rate of 0.3 mL min−1. Fortified recoveries in grape and soil samples ranged from 76.0% to 91.9% and relative standard deviations were less than 11.4% with fortified levels of 0.025–1.0 mg kg−1. The limits of detection and quantification were 0.005 mg kg−1 and 0.025 mg kg−1, respectively, with linear calibration curves extending up to 5.0 mg kg−1. The field experimental results showed that dissipations of epoxiconazole stereoisomers in grape followed first-order kinetics (R2 > 0.92) and stereoselectivity occurred in 2 h after spraying. The (−)-stereoisomer with half-life of 9.3 d degraded faster than (+)-stereoisomer with that of 13.2 d, and resulted in relative enrichment of (+)-stereoisomer. However, the stereoisomeric dissipations in soil were triphasic (“increase–decrease–steady”) with lower dissipation rates, and also occurred with preferential degradation of (−)-stereoisomer under field condition. The results for stereoselective dissipations can be applied for food and environmental assessments of chiral pesticides.
► Stereoselective dissipation of epoxiconazole under field condition was investigated.
► A simple and rapid chiral LC–MS/MS method was established for analysis of epoxiconazole.
► Significant stereoselective dissipation of epoxiconazole was observed in grape and soil.
► (−)-Stereoisomer of epoxiconazole degraded faster than (+)-stereoisomer both in grape and soil.
Journal: Chemosphere - Volume 87, Issue 8, May 2012, Pages 982–987