کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4412381 | 1307636 | 2009 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: In vivo evaluation of oxidative stress biomarkers in the land snail, Theba pisana exposed to copper-based pesticides In vivo evaluation of oxidative stress biomarkers in the land snail, Theba pisana exposed to copper-based pesticides](/preview/png/4412381.png)
The in vivo evaluation of oxidative stress biomarkers in the digestive gland of Theba pisana exposed to sublethal doses (40% and 80% of LD50 after 48 h) of copper-based pesticides; copper oxychloride, copper hydroxide and copper sulphate was examined. Oxidative individual perturbations were assessed by measuring non-enzymatic (glutathione; GSH) and enzymatic (catalase; CAT, glutathione peroxidase; GPx and glutathione S-transferase; GST) antioxidants in digestive gland of the snails. Lipid peroxidation (LPO) was also evaluated as marker of cell damage. The results indicated that copper sulphate was the most potent compound against this snail followed by copper hydroxide and copper oxychloride where their corresponding LD50 values were 26.54, 334.54 and 582.18 μg snail−1, respectively. Copper-based compounds resulted in a significant increase in the level of LPO whereas a significant decline of GSH content in the digestive gland of snails was observed when compared with untreated controls. The CAT, GPx and GST activities of treated snails were significantly higher than those of untreated controls. In general, the activation power of these compounds was in the following order: copper sulphate > copper hydroxide > copper oxychloride. This study suggests that up-regulation of the antioxidant enzyme activities, elevation of LPO and the reduction of GSH content is related to oxidative stress in this species that they could be used as potential biomarkers of copper-based pesticides exposure.
Journal: Chemosphere - Volume 77, Issue 3, October 2009, Pages 339–344