کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4413008 1307660 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Uptake visualization of deltamethrin by NanoSIMS and acute toxicity to the water flea Daphnia magna
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
Uptake visualization of deltamethrin by NanoSIMS and acute toxicity to the water flea Daphnia magna
چکیده انگلیسی

The objective of this study was to investigate the uptake of deltamethrin, an insecticide, by Daphnia magna neonates by SIMS and to compare these findings with results based on established toxicity tests.Young daphnids (aged <24 h) were exposed to 0, 50 and 200 μg L−1 (ppb) deltamethrin. Mobile, immobile and dead animals were enumerated after 24 and 48 h following OECD 202 [OECD 202, 2004. Daphnia sp., acute immobilisation test, guideline for testing of chemicals] guidelines. The animals were embedded in epoxy resin, cut into semi-thin sections (500 nm) and placed on silicon supporters. NanoSIMS 50 (Cameca) images were made from tissues of the intestine for carbon, nitrogen (measured as CN), phosphorus and bromine. To distinguish between relative concentrations of bromine in the guts from different exposure concentrations of deltamethrin, a carbon normalization method was carried out.Both deltamethrin concentrations and time showed a significant effect on immobilization and mortality of the daphnids (P < 0.0001). Bromine from deltamethrin could be visualized by NanoSIMS in all exposed gut tissues (gut wall, microvilli layer, perithropic membrane). Highest deltamethrin concentrations following 12C normalization were found in animals exposed to 200 μg L−1 deltamethrin, followed by 50 μg L−1 and the control.NanoSIMS 50 was successfully used as a supplemental technique for elucidating the relation between the uptake and localization of deltamethrin and its toxicity to D. magna. These results highlight the potential usefulness of NanoSIMS to detect marker elements of xenobiotic compounds within exposed organisms, to compare relative exposure concentrations, and to locate these compounds at their original tissue location.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemosphere - Volume 76, Issue 1, June 2009, Pages 134–140
نویسندگان
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