کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4413353 | 1307674 | 2009 | 7 صفحه PDF | دانلود رایگان |

Enantioselectivity in the separation, toxicology, biodegradation and estrogenic activity of chiral pesticides has become a groundbreaking topic recently. In this study, real-time, quantitative polymerase chain reaction was adapted to investigate the induction of estrogen-responsive gene expression in embryo-larval zebrafish after 7 d of exposure to permethrin (PM) enantiomers. The PM enantiomers were completely separated by a chiral HPLC column. The in vivo study found that a 7 d exposure to 250 ng L−1 PM racemate and its enantiomers was sufficient to stimulate vtg1, esrα and cyp19b expression, while 1000 ng L−1 exposure significantly induced gene expression in a pattern similar to that of the control (50 ng L−1 E2), except for vtg2. Significant differences were detected between the enantiomers in the induction of estrogen-responsive gene expression. At the exposure level of 1000 ng L−1, the vtg1, esrα and cyp19b responses to the (−)-trans enantiomer were about 3.2-, 1.8- and 1.5-fold higher, respectively, than those in the group treated with (+)-trans enantiomer (p < 0.05). In the two cis-enantiomer treatment groups, (+)-cis increased the mRNA level of the cyp19b gene about 1.5-fold higher than the (−)-cis-enantiomer did. Of the four enantiomers, the (−)-trans enantiomer showed the greatest estrogenic activity. The results strongly indicate the occurrence of significant enantioselectivity in estrogenic activity of PM enantiomers exposed to embryo-larval zebrafish. These findings add to a growing body of evidence concerning enantioselectivity in the toxicity, endocrine-disrupting activity, and environmental biodegradation of chiral pesticides.
Journal: Chemosphere - Volume 74, Issue 9, March 2009, Pages 1238–1244