کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4415377 1307745 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
AhR-mediated and antiestrogenic activity of humic substances
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
AhR-mediated and antiestrogenic activity of humic substances
چکیده انگلیسی

Humic substances (HS) were for decades regarded as inert in the ecosystems with respect to their possible toxicity. However, HS have been recently shown to elicit various adverse effects generally attributed to xenobiotics. In our study, we used MVLN and H4IIE-luc cell lines stably transfected with luciferase gene under control of estrogen receptor (ER) and Ah receptor (AhR; receptor connected with so-called dioxin-like toxicity) for assessment of anti/estrogenic and AhR-mediated effects of 12 commercially available humic substances. Out of those, five humic acids were shown to induce AhR-mediated activity with relative potencies related to TCDD 2.6 × 10−8–7.4 × 10−8. Organic extracts of HS solutions also elicited high activities what means that lipophilic molecules are responsible for a great part of effect. However, relatively high activity remaining in extracted solution suggests also presence of polar AhR-agonists. Contribution of persistent organic compounds to the observed effects was ruled out by H2SO4 treatment. Eight out of twelve HS elicited significant antiestrogenic effects with IC50 ranging from 40 to 164 mg l−1. The possible explanations of the antiestrogenic effect include sorption of 17-β-estradiol (E2) on HS, changes in membrane permeability for E2 or another specific mechanism.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemosphere - Volume 67, Issue 6, April 2007, Pages 1096–1101
نویسندگان
, , , , , ,