کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4416618 1307791 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Chemical and biological oxidative effects of carbon black nanoparticles
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
Chemical and biological oxidative effects of carbon black nanoparticles
چکیده انگلیسی

Several studies show that ultrafine particles have a larger surface area than coarse particles, thus causing a greater inflammatory response. In this study, we investigated chemical and biological oxidative effects of nanoparticles in vitro. Carbon black (CB) nanoparticles with mean aerodynamic diameters of 14, 56, and 95 nm were examined. The innate oxidative capacity of the CB nanoparticles was measured by consumption of dithiothreitol (DTT) in cell-free system. The expression of heme oxygenase-1 (HO-1) in rat alveolar type II epithelial cell line (SV40T2) and alveolar macrophages (AM) exposed to CB nanoparticles was measured by ELISA. DTT consumption of 14 nm CB was higher than that of other CB nanoparticles having the same particle weight. However, DTT consumption was directly proportional to the particle surface area. HO-1 protein in SV40T2 cells was significantly increased by the 14 nm and 56 nm CB, however, 95 nm CB did not affect. HO-1 protein in AM was significantly increased by the 14, 56, and 95 nm CB. The increase in HO-1 expression was diminished by N-acetyl-l-cysteine (NAC) treatment of each CB nanoparticles before exposure although the difference between the effects of NAC-treated and untreated 14 nm CB did not achieve significant. In conclusion, CB nanoparticles have innate oxidative capacity that may be dependent on the surface area. CB nanoparticles can induce oxidative stress in alveolar epithelial cells and AM that is more prominent with smaller particles. The oxidative stress may, at least partially, be mediated by surface function of particles.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemosphere - Volume 65, Issue 6, November 2006, Pages 946–951
نویسندگان
, ,