کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4433049 1619946 2007 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Application of real-time quantitative PCR for the detection of selected bacterial pathogens during municipal wastewater treatment
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
Application of real-time quantitative PCR for the detection of selected bacterial pathogens during municipal wastewater treatment
چکیده انگلیسی

Bacteria were detected at five stages of municipal wastewater treatment using TaqMan® real-time quantitative PCR (qPCR). Thirteen probe and primer sets were tested for diverse pathogens that may be present in wastewater, including Aeromonas hydrophila, Bacillus cereus, Clostridium perfringens, Enterococcus faecalis, Escherichia coli, E. coli O157:H7, Helicobacter pylori, Klebsiella pneumoniae, Legionella pneumophila, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella sp., and Staphylococcus aureus. The sensitivity of the assay was 100 fg of genomic DNA (= 22 gene copies), based on a standard curve generated using A. hydrophila purified DNA. Samples from five stages of wastewater treatment were collected, including raw wastewater, primary effluents, mixed liquor, waste activated sludge and final effluents. In duplicate samples, E. coli, K. pneumoniae, C. perfringens and E. faecalis were detected throughout the wastewater process, and their numbers decreased by 3.52–3.98, 4.23–4.33, 3.15–3.39, and 3.24 orders of magnitude respectively, between the raw wastewater and final effluent stage. This qPCR method was effective for the detection of pathogens in wastewater and confirmed that the risk of exposure to pathogens in the wastewater discharge was well within the Environment Canada guidelines.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Science of The Total Environment - Volume 382, Issue 1, 15 August 2007, Pages 121–129
نویسندگان
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