کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4482344 | 1316856 | 2012 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Genetic studies of the role of fatty acid and coenzyme A in photocatalytic inactivation of Escherichia coli Genetic studies of the role of fatty acid and coenzyme A in photocatalytic inactivation of Escherichia coli](/preview/png/4482344.png)
The roles of bacterial cellular components, namely, fatty acid profile and coenzyme A, in photocatalytic inactivation of bacteria were investigated. Escherichia coli BW25113, as a “parental strain”, and its isogenic single-gene deletion mutants E. coli JW1081 (fabF− mutant) and E. coli JW3942 (coaA− mutant) showed different susceptibilities towards photocatalytic inactivation by titanium dioxide (TiO2, irradiated by UVA lamps (λ = 365 nm)). Regulating the fatty acid composition through pre-incubation temperature adjustment demonstrated the crucial role of cell membrane fatty acid profile in bacterial susceptibility towards photocatalytic inactivation, while the lower coenzyme A level in coaA− mutant correlated well with its lower susceptibility towards photocatalytic inactivation. Furthermore, transmission electron microscopic study demonstrated the photocatalytic destruction process of bacterial cells. This is the first study using single-gene deletion mutants to explore better understanding of the photocatalytic inactivation mechanism of E. coli.
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► The isogenic fabF− mutant is slightly more susceptible to photocatalytic inactivation.
► The isogenic coaA− mutant is less susceptible to photocatalytic inactivation.
► Temperature regulates the ratio of unsaturated to saturated fatty acid (FA) of cell membrane.
► FA profile and coenzyme A level significantly affect photocatalytic inactivation of bacteria.
Journal: Water Research - Volume 46, Issue 13, 1 September 2012, Pages 3951–3957