کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4483826 1316900 2010 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Metabolic turnover and catalase activity of biofilms of Pseudomonas fluorescens (ATCC 17552) as related to copper corrosion
موضوعات مرتبط
مهندسی و علوم پایه علوم زمین و سیارات فرآیندهای سطح زمین
پیش نمایش صفحه اول مقاله
Metabolic turnover and catalase activity of biofilms of Pseudomonas fluorescens (ATCC 17552) as related to copper corrosion
چکیده انگلیسی

In this work we report the results of a combined biochemical and electrochemical study aimed to analyze both the growth of biofilms of Pseudomonas fluorescens on copper samples and its possible role in the instability of the metal/electrolyte interface.DNA and RNA were quantified along the time for biofilms grown on copper and glass to estimate both the growth of the bacterial population and its metabolic state (through the RNA/DNA ratio). The expression and specific activity of catalase were also determined to gain insight into their possible role in corrosion acceleration. The electrochemical behavior of the biofilm/copper interface was monitored by Linear Polarization Resistance (Rp) and electrochemical impedance spectroscopy (EIS) along the experiments.Results showed a longer lag phase for biofilms developing on copper that included a period of high metabolic activity (as measured by the RNA/DNA ratio) without biomass growth. Biological activity introduced a new time constant at intermediate frequencies in EIS spectra whose capacitive behavior increased with the biofilm development. The increment in this biofilm-related signal was accompanied by a strong limitation to charge transfer through a diffusion controlled process probably due to oxygen exhaustion by cells respiration, while the resistance of the interface decreased presumably due to oxide dissolution by local acidification under the colonies. In addition, catalase activity was found to be high in mature copper-tolerant biofilms, which differentially express a catalase isoform not present in biofilms growing on glass.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Water Research - Volume 44, Issue 8, April 2010, Pages 2592–2600
نویسندگان
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