Free chlorine demand and cell survival of microbial suspensions

The utility of chlorine residual and chlorine demand as a surrogate for microbial contamination in the water distribution system was evaluated. The chlorine demanded by and cell survival of pure culture suspensions of Escherichia coli, Staphylococcus epidermidis, and Mycobacterium aurum were quantified in solutions with initial free chlorine concentrations of 0.20, 0.40, and 0.80 mg/L. The chlorine demand increased with initial concentration of cells and free chlorine for all species. At equivalent initial cell concentrations, chlorine demand was greatest for M. aurum, followed by S. epidermidis and E. coli. The chlorine contact time required for a 3-log inactivation of E. coli, S. epidermidis, and M. aurum was calculated as 0.032±0.009, 0.221±0.080, and 42.9±2.71 mg min/L, respectively. The ultimate chlorine demand and cell survival were directly proportional. No chlorine demand was observed at cell concentrations less than 105 CFU/mL for E. coli or 104 CFU/mL for S. epidermidis. M. aurum demanded chlorine at all initial cell concentrations including 103 CFU/mL, which was the detection limit of the cell quantification assay. Chlorine demand was determined to be a suitable surrogate indicator of the organisms studied and its utility may be enhanced in locations of the water distribution system that maintain a higher free chlorine residual.