کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4486135 1316976 2006 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Natural and synthetic hormone removal using the horseradish peroxidase enzyme: Temperature and pH effects
موضوعات مرتبط
مهندسی و علوم پایه علوم زمین و سیارات فرآیندهای سطح زمین
پیش نمایش صفحه اول مقاله
Natural and synthetic hormone removal using the horseradish peroxidase enzyme: Temperature and pH effects
چکیده انگلیسی

The primary objective of our research was to establish the technical feasibility of using the horseradish peroxidase (HRP) enzyme for natural and synthetic estrogens–estrone (E1), 17β-estradiol (E2), estriol (E3), and 17α-ethinylestradiol (EE2)–removal. The effects of temperature and pH on enzymatic treatment kinetics were investigated. Residual estrogen concentrations were quantified by liquid chromatography, coupled with mass spectrometry analysis. In a synthetic solution at pH 7 and 25±1 °C, the HRP enzyme-catalyzed process was capable of achieving 92–100% removal of E1, E2, E3, and EE2 within 1 h of treatment with an HRP activity of 0.017 U/ml. The influence of the pH (5–9) and temperature (5–35 °C) on estrogen removal was observed to be significant, with the optimum pH near neutral conditions. The results also showed that wastewater constituents significantly impact the HRP-catalyzed estrogen removal. The experimental research proved that the HRP-catalyzed system is technically feasible for the removal of the main estrogens present in the environment at low concentrations.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Water Research - Volume 40, Issue 15, August 2006, Pages 2847–2856
نویسندگان
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