Improvement of physical stability properties of kenaf (Hibiscus cannabinus L.) seed oil-in-water nanoemulsions

• Physical stability of kenaf seed oil-in-water nanoemulsion was improved by optimising three-component emulsifier mixtures that produced using high pressure homogeniser.
• The optimum proportions of emulsifier mixture obtained was 64.9% (w/w) SC, 6.4% (w/w) GA, and 28.7% (w/w) T20.
• The optimum ternary emulsifier mixtures showed a good synergistic stabilising effect.
• The optimised formulation was stable at both chill (4 °C) and room temperature (25 °C) over 1 month of evaluation.

Kenaf seed oil-in-water nanoemulsions were optimised using simplex centroid mixture design with three components (sodium caseinate, gum Arabic and Tween 20). In addition, the main, binary and ternary interaction effects among these three selected emulsifiers on physical stability were also studied. The mixture design showed a good fit to the predicted model with R2 > 0.89, 0.82, and 0.73 for mean particle size, polydispersity index (PDI) and zeta-potential, respectively. The optimum proportion of emulsifier mixtures was 64.9% (w/w) SC, 6.4% (w/w) GA, and 28.7% (w/w) T20 that predicted to produce mean particle size of 126.82 nm, PDI of 0.16 and zeta-potential of −43.47 mV. The experimental value obtained was 121.22 nm, 0.16 and −39.63 mV for mean particle size, PDI, and zeta-potential, respectively. No significant difference (p > 0.05) between the experimental and predicted values, indicating the suitability of the mixture design for optimising and developing stable kenaf seed oil-in-water nanoemulsions. The optimised formulation was stable at both chill (4 °C) and room temperature (25 °C) over 1 month of evaluation. The results have important implications for the development of stable kenaf seed oil-based nutraceutical products. It can be added into beverages such as dairy products to improve the nutrition value of the beverage.

Schematic diagram of producing kenaf seed oil-in-water nanoemulsions.Figure optionsDownload as PowerPoint slide