کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4516404 1322356 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A specific qualitative and real-time PCR detection of MON863 maize based on the 5′-transgene integration sequence
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
A specific qualitative and real-time PCR detection of MON863 maize based on the 5′-transgene integration sequence
چکیده انگلیسی

Genetically modified crops are widely grown in the world today. Labeling is required when genetically modified organisms (GMOs) are placed on the market. There is a need to establish a specific method for the detection of genetically modified foods. MON863 transgenic maize containing a Cry3Bb1 sequence that produces insecticidal protein cry3Bb1 is a major GMO crop. In this paper, we report studies that designed specific PCR primers and TaqMan probes based upon the 5′-transgene integration sequence, and developed qualitative and quantitative PCR conditions using these primers and probes. We determined the 5′-transgene integration sequence using a ligation-mediated polymerase chain reaction (LM PCR) method. In qualitative PCR studies, the limit of detection (LOD) was 0.5% for MON863 in 100 ng genomic DNA. In the quantitative PCR assays, the limit of detection (LOD) and limit of quantitation (LOQ) are 10 and 100 haploid copies, respectively. Maize samples with different contents of genetically modified component were tested using the established TaqMan real-time PCR system.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Cereal Science - Volume 48, Issue 3, November 2008, Pages 592–597
نویسندگان
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