کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4524541 1323581 2014 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Different genomic DNA methylation patterns between male and female adults of white-backed planthoppers Sogatella furcifera
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Different genomic DNA methylation patterns between male and female adults of white-backed planthoppers Sogatella furcifera
چکیده انگلیسی


• Genome methylation patterns are different between male and female adults.
• The fully-methylated ratio and hemi-methylated ratio in male are 6.88% and 11.19%.
• The fully-methylated ratio and hemi-methylated ratio in female are 7.64% and 8.00%.
• We found 20 male-specific methylated bands and 26 female-specific methylated bands.

DNA methylation plays a key role in gene regulation and phenotype variation in many organisms. The aim of this study was to survey the frequency and variation of cytosine methylation at CCGG sequences in adult male and female planthoppers Sogatella furcifera, a major rice pest in Asia, and to determine the occurrence of methylation changes associated with sexual dimorphism using methylation-sensitive amplification polymorphism. 1131 DNA fragments including CCGG sites were amplified using 36 pairs of selective primers: about 191 methylated bands were identified. In male planthoppers, we got a total of 581 bands, including 40 fully-methylated bands, 65 hemi-methylated bands and 476 none-methylated bands, so the fully-methylated ratio, hemi-methylated ratio and total methylated ratio were 6.88%, 11.19% and 18.07%, respectively. In the female planthopper, there were a total of 550 bands, including 44 fully-methylated bands, 42 hemi-methylated bands and 464 none-methylated bands. The fully-methylated ratio was 7.64% in female planthoppers, which was slightly higher than in the male planthoppers, however, the hemi-methylated ratio was lower (8.00%) in the female compared with the male planthopper. Altogether, 46 DNA bands displayed variable cytosine methylation patterns between male and female samples: 20 of them occurred only in male samples and 26 only in female samples. Thus, the genome methylation patterns are different between male and female adults. The results suggest that DNA methylation might be related to sexual differentiation and development in S. furcifera.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Asia-Pacific Entomology - Volume 17, Issue 4, December 2014, Pages 917–921
نویسندگان
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