کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4524779 1323592 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular cloning and antibacterial activity of bombolitin isolated from the venom of a bumblebee, Bombus terrestris
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Molecular cloning and antibacterial activity of bombolitin isolated from the venom of a bumblebee, Bombus terrestris
چکیده انگلیسی

Three major components of bumblebee venom are bombolitin, phospholipase A2, and a serine protease, with bombolitin being the most abundant. Here, we describe the molecular cloning of bombolitin isolated from the venom of a bumblebee, Bombus terrestris, and demonstrate its antibacterial activity. The B. terrestris bombolitin gene consists of 2 exons encoding 56 amino acid residues. Comparative analysis shows that mature B. terrestris bombolitin consists of 18 amino acid residues, which are identical to those of B. ignitus bombolitin. B. terrestris bombolitin displayed antibacterial activity against both the Gram-negative bacterium Klebsiella pneumoniae and the Gram-positive bacterium Staphylococcus aureus, indicating that B. terrestris bombolitin may be a potential antimicrobial agent.


► Bombus terrestris bombolitin consists of 2 exons encoding 56 amino acid residues.
► Bombolitin is expressed in the venom gland and cleaved to form an 18-amino acid mature peptide.
► B. terrestris bombolitin shows antibacterial activity against both the Gram-negative and Gram-positive bacterium.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Asia-Pacific Entomology - Volume 15, Issue 1, March 2012, Pages 21–25
نویسندگان
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