Inhibition of non-toxigenic Aspergillus niger FS10 isolated from Chinese fermented soybean on growth and aflatoxin B1 production by Aspergillus flavus

A non-toxigenic strain FS10 was isolated from Chinese fermented soybean to control AFB1 contamination and conformed as Aspergillus niger by 18S rDNA. To elucidate the biocontrol mechanism, the ability of culture filtrate of strain FS10 against growth and AFB1 production by toxigenic Aspergillus flavus was evaluated in vitro. Meanwhile, the influence of filtrate on spore morphology of A. flavus was analyzed by optical microscope and atomic force microscope (AFM). Results demonstrated that the culture filtrate of FS10 significantly suppressed A. flavus growth, sporulation and AFB1 biosynthesis. After 9 days, the inhibition of mycelia growth, sporulation and AFB1 production reached to 37.7%, 100% and 94.5%, respectively. In addition, optical microscopy and AFM showed that the culture filtrate serious damaged spore morphology, accompanying with spore analysis and cell wall collapse, resulted in inhibition of spore germination. The degradation activity of FS10 culture filtrate on AFB1 was also estimated. When AFB1 was incubated with the culture filtrate, 85.8% of AFB1 could be degraded after 5 days. It is concluded that the culture filtrate of strain FS10 has pleiotropic effects on control AFB1 contamination. As a safe, effective and economic biological agent, the filtrate could be applied in fermented food to prevent toxigenic Aspergillus spp. and AFB1 contamination.

► Aspergillus niger isolated from fermented soybean can control AFB1 contamination.
► The culture filtrate of A. niger inhibits Aspergillus flavus growth.
► The culture filtrate of A. niger inhibits AFB1 biosynthesis.
► The culture filtrate of A. destroys Aspergillus flavus spore.
► The culture filtrate of A. niger degrades AFB1.