کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4559718 | 1330471 | 2011 | 11 صفحه PDF | دانلود رایگان |

An acetylcholinesterase (AChE) B multisensor from Nippostrongylus brasiliensis (Nb) was developed, able to detect the most frequently used insecticides in Brazil. The objective was to establish a fast screening-out method, separating the negative samples from the positive ones. The four mutants, which together presented the widest sensitivity spectrum, were: F345A, M301A, W346V and W346A. The combination of these four mutants in a multienzyme biosensor array enabled the detection of 11 out of the 12 most important insecticides at concentrations below 10 μg/kg. The biosensor test was compared with traditional analysis methods, and validated with food samples previously analyzed. The storage stability revealed that the enzyme activity remained stable for 40 weeks; however the sensitivity decreased with time. The biosensor screened out samples with an analysis duration of about 2 h.
Journal: Food Control - Volume 22, Issue 7, July 2011, Pages 1061–1071