کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4559809 1330476 2011 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comparison between thiosulphate-citrate-bile salt sucrose (TCBS) agar and CHROMagar Vibrio for isolating Vibrio parahaemolyticus
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Comparison between thiosulphate-citrate-bile salt sucrose (TCBS) agar and CHROMagar Vibrio for isolating Vibrio parahaemolyticus
چکیده انگلیسی

Considering its widespread distribution in marine environments, its fast replication times and low infectious doses and the rapid spread of its strains in recent years, intensive and continuous monitoring of potentially pathogenic Vibrio parahaemolyticus is strongly recommended in order to assess the human health risk arising from shellfish consumption. The lack of epidemiological data points to the need to develop specific methods for detecting V. parahaemolyticus. In this note, the authors compare two plating media currently available for isolating V. parahaemolyticus in shellfish. Both approaches involve pre-enrichment of V. parahaemolyticus. One uses thiosulphate-citrate-bile salt sucrose (TCBS) as the isolation medium, while the other uses a chromogenic medium (CHROMagar Vibrio). Next, biochemical identification of isolates was performed with API 20E, followed by PCR assay aimed at the toxR gene to confirm the cultural and biochemical identification. Comparison of the two methods highlighted that CHROMagar Vibrio is more accurate and specific than TCBS. The analysis of data from 160 shellfish samples showed an accuracy and specificity of just 51% and 71% for TCBS compared with 88% and 95% for CAV.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Control - Volume 22, Issue 1, January 2011, Pages 124–127
نویسندگان
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