کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4559898 1330480 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantification of Lactobacillus gasseri, Enterococcus faecium and Bifidobacterium infantis in a probiotic OTC drug by real-time PCR
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Quantification of Lactobacillus gasseri, Enterococcus faecium and Bifidobacterium infantis in a probiotic OTC drug by real-time PCR
چکیده انگلیسی

Application of real-time PCR and SYBR Green for selective enumeration of probiotic bacteria in a lyophilised probiotic product sold as an OTC drug, containing Lactobacillus gasseri, Bifidobacterium infantis and Enterococcus faecium was evaluated. Maxwell DNA Purification Kit was found the most efficient and repeatable. Among nine pairs of primers tested, the following were selected: Enc-F, Enc-R for E. faecium, Lgas-3/Lgas-2 for L. gasseri and BiINF-1/BiINF-2 for B. infantis. Comparison of plate counts and real-time PCR analysis of 10 batches of product showed that on average 62% of lactobacilli, 45% of enterococci and 2% of bifidobacteria preserved their culturability.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Control - Volume 21, Issue 4, April 2010, Pages 419–425
نویسندگان
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