کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4560373 | 1330502 | 2008 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Multiplex qualitative PCR assay for identification of genetically modified canola events and real-time event-specific PCR assay for quantification of the GT73 canola event
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Multiplex qualitative PCR assay for identification of genetically modified canola events and real-time event-specific PCR assay for quantification of the GT73 canola event Multiplex qualitative PCR assay for identification of genetically modified canola events and real-time event-specific PCR assay for quantification of the GT73 canola event](/preview/png/4560373.png)
چکیده انگلیسی
Genetically modified (GM) canola is the most widely grown oilseed crop in Canada. At this time, commercially produced GM canola cultivars in Canada have the events GT73/RT73 and Ms8xRf3. Commercial seed sale of canola cultivars containing the GM events such as OXY235 and T45 has been discontinued. Adventitious presence of GM seeds and grains in non-GM grains is a concern for international grain trade, and development of effective detection methods is important. A multiplex qualitative PCR procedure was established for the detection of the GM canola events OXY235, Ms8xRf3, T45 and GT73. The presence of the GM canola events was also successfully detected in ground spiked wheat and barley grain samples prepared at 0.1%, 0.5% and 1.0% levels (w/w). The GT73 real-time PCR assay was successfully used to quantify DNA extracted from spiked ground canola samples consisting of 5%, 1%, 0.5% and 0.1% GT73 (w/w).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Control - Volume 19, Issue 9, September 2008, Pages 893-897
Journal: Food Control - Volume 19, Issue 9, September 2008, Pages 893-897
نویسندگان
Tigst Demeke, Indira Ratnayaka,