Determination of water content in bee’s pollen samples by Karl Fischer titration

It was analyzed the water content of three pollen samples from three different sources, using the classical chemical method (Karl Fischer). It is very important for this method to select the proper work conditions because pollen has a complex composition. For Karl Fischer’s method we selected the most adequate solvents, temperature and particle size. The Karl Fischer titration in a mixture of solvents (Hydranal® solvent and n-octanol) at high temperature (50 °C) is the most indicated for the determination of water content in these products.