کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4562546 | 1330720 | 2010 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Comparative spectroscopic and rheological studies on crude and purified soluble barley and oat β-glucan preparations Comparative spectroscopic and rheological studies on crude and purified soluble barley and oat β-glucan preparations](/preview/png/4562546.png)
The rheology of crude and purified barley (BBG) and oat (OBG) β-glucan samples were characterized. Sample content and major impurities was characterized by Fourier-transform near infrared Raman and infrared (FT-IR) spectroscopy revealing substantial differences between the β-glucan samples. The purification procedure increased the β-glucan content from 66.7 to 82.4% and from 30.1 to 68.4% for BBG and OBG, respectively. Proton nuclear magnetic resonance (1H NMR) analysis was applied to estimate the β-(1 → 3) to β-(1 → 4) linkage ratio of the β-glucans. The molar mass of BBG and OBG was determined by high performance size-exclusion chromatography (HPSEC) using β-glucan standards and was found to be 126 and 355 kDa, respectively.The viscosity of crude and purified β-glucans was studied at various concentrations (2.5, 5% w/v), temperatures (10–80 °C) and shear rates (1–100 s− 1). BBG was characterized as a low-viscosity β-glucan with Newtonian flow behavior while OBG was characterized as a high-viscosity β-glucan with shear thinning flow behavior. At equivalent β-glucan concentration in solutions the viscosity for OBG was found to be ~ 100 fold higher than for BBG. A direct viscosity dependence on exact β-glucan content regardless of amount and composition of α-glucan impurities was found for both OBG and BBG. This study suggests that the structural characteristics of the β-glucan polymers such as molar mass are of greater functional importance than the presence of lager amounts of starch/α-dextrins as long as the β-glucan samples are compared at equivalent β-glucan doses.
Journal: Food Research International - Volume 43, Issue 10, December 2010, Pages 2417–2424