کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4753292 | 1416551 | 2017 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Repression of the DCL2 and DCL4 genes in Nicotiana benthamiana plants for the transient expression of recombinant proteins
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The production of recombinant proteins in plants has many advantages, including safety and reduced costs. However, this technology still faces several issues, including low levels of production. The repression of RNA silencing seems to be particularly important for improving recombinant protein production because RNA silencing effectively degrades transgene-derived mRNAs in plant cells. Therefore, to overcome this, we used RNA interference technology to develop DCL2- and DCL4-repressed transgenic Nicotiana benthamiana plants (ÎD2, ÎD4, and ÎD2ÎD4 plants), which had much lower levels of NbDCL2 and/or NbDCL4 mRNAs than wild-type plants. A transient gene expression assay showed that the ÎD2ÎD4 plants accumulated larger amounts of green fluorescent protein (GFP) and human acidic fibroblast growth factor (aFGF) than ÎD2, ÎD4, and wild-type plants. Furthermore, the levels of GFP and aFGF mRNAs were also higher in ÎD2ÎD4 plants than in ÎD2, ÎD4, and wild-type plants. These findings demonstrate that ÎD2ÎD4 plants express larger amounts of recombinant proteins than wild-type plants, and so would be useful for recombinant protein production.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 124, Issue 2, August 2017, Pages 215-220
Journal: Journal of Bioscience and Bioengineering - Volume 124, Issue 2, August 2017, Pages 215-220
نویسندگان
Kouki Matsuo, Takeshi Matsumura,