کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4753486 | 1416980 | 2017 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Research paperDNA-guided assembly of a five-component enzyme cascade for enhanced conversion of cellulose to gluconic acid and H2O2 Research paperDNA-guided assembly of a five-component enzyme cascade for enhanced conversion of cellulose to gluconic acid and H2O2](/preview/png/4753486.png)
- Direct conversion of cellulose to gluconic acid and H2O2 using a DNA-guided five-enzyme cascade.
- Enhanced H2O2 production by over 11-fold using the assembled BlgA and GOX cascade.
- Enhanced H2O2 production using the assembled five-enzyme cascade.
Enzymatic fuel cells have received considerable attention because of their potential for direct conversion of abundant raw materials such as cellulose to electricity. The use of multi-enzyme cascades is particularly attractive as they offer the possibility of achieving a series of complex reactions at higher efficiencies. Here we reported the use of a DNA-guided approach to assemble a five-component enzyme cascade for direct conversion of cellulose to gluconic acid and H2O2. Site-specific co-localization of β-glucosidase and glucose oxidase resulted in over 11-fold improvement in H2O2 production from cellobiose, highlighting the benefit of substrate channeling. Although a more modest 1.5-fold improvement in H2O2 production was observed using a five-enzyme cascade, due to H2O2 inhibition on enzyme activity, these results demonstrated the possibility to enhance the production of gluconic acid and H2O2 directly from cellulose by DNA-guided enzyme assembly.
Journal: Journal of Biotechnology - Volume 263, 10 December 2017, Pages 30-35