کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4760873 | 1422471 | 2017 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Simultaneous quantification of batrachotoxin and epibatidine in plasma by ultra-performance liquid chromatography/tandem mass spectrometry
ترجمه فارسی عنوان
اندازه گیری همزمان باتراکوتوکسین و اپیباتیدین در پلاسمای با استفاده از کروماتوگرافی مایع فوق العاده / اسپکترومتری جرم دو طرفه
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
An ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the simultaneous quantification of batrachotoxin and epibatidine in plasma. Plasma samples were pretreated by liquid-liquid extraction with acetonitrile and methanol. The toxins were separated on a reversed phase C18-column (2.1 mm Ã 50 mm, 1.7 μm) using a formic acid/acetonitrile gradient elution. Quantification was carried out by mass chromatography with each product ion referenced against midazolam-d4 as an internal standard (IS). The two toxins and the IS were separated within 2 min. The calibration curves for the two toxins spiked into human plasma showed good linearities in the range from 2.5 to 250 ng/mL. The detection limits were estimated to be 0.5 ng/mL for batrachotoxin and 1 ng/mL for epibatidine with a signal-to-noise ratio of 3:1. Overall recoveries ranged from 69.6% to 98.2%, and no significant matrix effects were observed. The intra- and interday accuracies were 94.7-102.3%, and the precisions were 1.0-10.3%. This method was successfully applied for the quantification of batrachotoxin and epibatidine in rat plasma samples taken after intraperitoneal administration of the toxins. This is the first report to use UPLC-MS/MS to simultaneously quantify batrachotoxin and epibatidine in plasma samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Legal Medicine - Volume 25, March 2017, Pages 1-5
Journal: Legal Medicine - Volume 25, March 2017, Pages 1-5
نویسندگان
Yosuke Shiraishi, Tadashi Ogawa, Takayoshi Suzuki, Masae Iwai, Maiko Kusano, Kei Zaitsu, Fumio Kondo, Akira Ishii, Hiroshi Seno,