Oxygen carrying capability evaluation based on direct electrochemistry of highly loaded hemoglobin spheres

As reported, the optimal size of hemoglobin-based oxygen carriers (HBOCs) should be preferably less than 1 µm and more than 100 nm. In this study, we employed manganese carbonate (MnCO3) for the production of preferably size multilayer hemoglobin (Hb) based sphere with glutaraldehyde (GA) as the crosslinking agent. By comparison, it can be found that {Hb/GA}5 also has a higher sensitivity to oxygen, stronger carrying-oxygen ability and more stable properties than that of {Hb/GA}1. However, MnCO3 based Hb-loaded spheres did not show better performance than CaCO3 based Hb-loaded spheres. 177