کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4997909 | 1459918 | 2017 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Exploring the potential of iTRAQ proteomics for tracking the transformation of extracellular proteins from enzyme-disintegrated waste activated sludge
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Exploring the potential of iTRAQ proteomics for tracking the transformation of extracellular proteins from enzyme-disintegrated waste activated sludge Exploring the potential of iTRAQ proteomics for tracking the transformation of extracellular proteins from enzyme-disintegrated waste activated sludge](/preview/png/4997909.png)
چکیده انگلیسی
To characterize the transformation of extracellular proteins extracted from raw and enzyme-disintegrated waste activated sludge (WAS), extracts of extracellular polymeric substances (EPS) were subject to isobaric tags for relative and absolute quantitation (iTRAQ) proteomics analysis. 209 proteins were identified and categorized into three Gene Ontology classifications: “cellular components”, “molecular function”, and “biological processes”. Most identified proteins originated from intracellular components, organelles, or cytoplasm, suggesting that cell decline and lysis represent the main sources of extracellular proteins in WAS. The major protein functions comprised “transporter binding activity”, “macromolecule metabolic process”, and “biosynthesis enzyme catalytic activity”. In total, 15 proteins categorized as “membrane part” and “biological adhesion” and 10 as “organelle” were down- or up-regulated, respectively, implying that the enzyme-disintegration mainly induced WAS floc-structure disintegration via membrane structure and corresponding biological adhesion disruption. The proteomics study will provide valuable clues to better understand EPS changes associated with enzymatic treatment at molecular levels.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioresource Technology - Volume 225, February 2017, Pages 75-83
Journal: Bioresource Technology - Volume 225, February 2017, Pages 75-83
نویسندگان
Boran Wu, Lianghu Su, Liyan Song, Xiaohu Dai, Xiaoli Chai,