Cost-effective simultaneous saccharification and fermentation of l-lactic acid from bagasse sulfite pulp by Bacillus coagulans CC17

- SSF demonstrated the remarkable advantage over SHF from BSP by strain CC17.
- SSF by strain CC17 could lower about 33.3% fungal cellulase dosage over SHF.
- Strain CC17 could convert cellobiose to lactic acid without exogenous β-glucosidase.
- 110 g/L l-lactic acid was obtained in the fed-batch SSF of BSP by strain CC17.

The main barriers to cost-effective lactic acid production from lignocellulose are the high cost of enzymes and the ineffective utilization of the xylose within the hydrolysate. In the present study, the thermophilic Bacillus coagulans strain CC17 was used for the simultaneous saccharification and fermentation (SSF) of bagasse sulfite pulp (BSP) to produce l-lactic acid. Unexpectedly, SSF by CC17 required approximately 33.33% less fungal cellulase than did separate hydrolysis and fermentation (SHF). More interestingly, CC17 can co-ferment cellobiose and xylose without any exogenous β-glucosidase in SSF. Moreover, adding xylanase could increase the concentration of lactic acid produced via SSF. Up to 110 g/L of l-lactic acid was obtained using fed-batch SSF, resulting in a lactic acid yield of 0.72 g/g cellulose. These results suggest that SSF using CC17 has a remarkable advantage over SHF and that a potentially low-cost and highly-efficient fermentation process can be established using this protocol.