کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5000 | 269 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evaluation of the use of volatile electrolyte system produced by ammonia and carbon dioxide in water for the salting-out of proteins: Precipitation of porcine trypsin
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Evaluation of the use of volatile electrolyte system produced by ammonia and carbon dioxide in water for the salting-out of proteins: Precipitation of porcine trypsin Evaluation of the use of volatile electrolyte system produced by ammonia and carbon dioxide in water for the salting-out of proteins: Precipitation of porcine trypsin](/preview/png/5000.png)
چکیده انگلیسی
In this work, the precipitation of porcine trypsin in solutions of volatile electrolytes formed by ammonia and carbon dioxide was investigated. The solubility curve of trypsin at 4 °C was determined by measuring the compositions of the precipitate and supernatant phases. Analysis showed that ammonium carbamate is able to induce trypsin salting-out. The verified salting-out property of ammonium carbamate, the easy removal of the salt out of the protein precipitate and mother-solution and the high activity recovery demonstrate the feasibility of using ammonium carbamate as precipitant agent.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 30, Issue 2, 1 June 2006, Pages 124–129
Journal: Biochemical Engineering Journal - Volume 30, Issue 2, 1 June 2006, Pages 124–129
نویسندگان
Erika Ohta Watanabe, Pedro de Alcântara Pessôa Filho, Everson Alves Miranda, Rahoma Sadeg Mohamed,