کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5030809 1470932 2018 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Flexible nanohybrid microelectrode based on carbon fiber wrapped by gold nanoparticles decorated nitrogen doped carbon nanotube arrays: In situ electrochemical detection in live cancer cells
ترجمه فارسی عنوان
میکرو الکترودهای نانویبرید انعطاف پذیر بر پایه فیبر کربن پیچیده شده توسط نانوذرات طلا، نانوذرات کربنی اعمال شده با نیتروژن تزئینی: تشخیص الکتروشیمیایی در سلولهای سرطانی زنده
کلمات کلیدی
میکرو الکترودهای فیبر انعطاف پذیر، آلیاژهای نیتروژن کربن نیتروژن، نانوذرات طلا، بیوسنسورهای الکتروشیمیایی، تشخیص در محل، سلول های سرطانی،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The rapidly growing demand for in situ real-time monitoring of chemical information in vitro and in vivo has attracted tremendous research efforts into the design and construction of high-performance biosensor devices. Herein, we develop a new type of flexible nanohybrid microelectrode based on carbon fiber wrapped by gold nanoparticles decorated nitrogen-doped carbon nanotube arrays, and explore its practical application in in situ electrochemical detection of cancer biomarker H2O2 secreted from live cancer cells. Our results demonstrate that carbon fiber material with microscale size and fascinating mechanical properties can be used as a robust and flexible microelectrode substrate in the electrochemical biosensor system. And the highly ordered nitrogen-doped carbon nanotube arrays that grown on carbon fiber possess high surface area-to-volume ratio and abundant active sites, which facilitate the loading of high-density and uniformly dispersed gold nanoparticles on it. Benefited from the unique microstructure and excellent electrocatalytic properties of different components in the nanohybrid fiber microelectrode, an effective electrochemical sensing platform based on it has been built up for the sensitive and selective detection of H2O2, the detection limit is calculated to be 50 nM when the signal-to-noise ratio is 3:1, and the linear dynamic range is up to 4.3 mM, with a high sensitivity of 142 µA cm−2 mM−1. These good sensing performances, coupled with its intrinsic mechanical flexibility and biocompatibility, allow for its use in in situ real-time tracking H2O2 secreted from breast cancer cell lines MCF-7 and MBA-MD-231, and evaluating the sensitivity of different cancer cells to chemotherapy or radiotherapy treatments, which hold great promise for clinic application in cancer diagnose and management.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 100, 15 February 2018, Pages 453-461
نویسندگان
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