کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5031220 1470942 2017 32 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Integration of isothermal amplification methods in microfluidic devices: Recent advances
ترجمه فارسی عنوان
یکپارچگی روش های تقویت کننده های ایزوترمال در دستگاه های میکرو فلوئیدیک: پیشرفت های اخیر
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The amplification of the target nucleic acid sequence represents a key step for the development of sensitive detection protocols. The integration in microfluidic devices of the most popular technology for nucleic acids amplifications, polymerase chain reaction (PCR), is significantly limited by the thermal cycling needed to obtain the target sequence amplification. This review provides an overview of recent advances in integration of isothermal amplification methods in microfluidic devices. Isothermal methods, that operate at constant temperature, have emerged as promising alternative to PCR and greatly simplify the implementation of amplification methods in point-of-care diagnostic devices and devices to be used in resource-limited settings. Possibilities offered by isothermal methods for digital droplet amplification are discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 90, 15 April 2017, Pages 174-186
نویسندگان
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