کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5031248 1470942 2017 20 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Impedimetric monitoring of apoptosis using cytochrome-aptamer bioconjugated silver nanocluster
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Impedimetric monitoring of apoptosis using cytochrome-aptamer bioconjugated silver nanocluster
چکیده انگلیسی
It is well known that cytochrome c (Cyt c) is a crucial death regulator that triggers programmed cell death, apoptosis. Here, we report on the successful application of an aptamer bioconjugated nanoclusters for the detection of apoptosis based on release of cytochrome c from mitochondria in lysates human embryonic kidney cells HEK293T. The aptamer-conjugated silver nanoclusters (Apt@AgNCs) were synthesized and immobilized on gold electrode for impedimetric detection of Cyt c over the range of 0.15-375 nM. It was found that the presence of Ag(I) ions shell at the surface of prepared NCs could increase their affinity to the donor groups (COO- and some amine groups in its deprotonated form, NH2) of cysteine attached on the gold electrode surface. The use of NCs, which play the role of conductive holders, established a simple immobilization procedure based on a low cost non-functionalized DNA sequence. To diminish the probable nonspecific impedance changes and decrease time of measurement, a rapid single frequency measurements (SFM) was assessed based on recording total impedance |Z| in different individual frequencies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 90, 15 April 2017, Pages 195-202
نویسندگان
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