کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5130617 | 1490846 | 2017 | 7 صفحه PDF | دانلود رایگان |
- An incorporated signal amplification strategy was proposed.
- Co3O4 nanoparticles exhibited multifunctional character.
- Au@MPTES-GS possesses high surface area and excellent conductivity.
- The proposed immunosensor exhibited a low detection limit of 0.029 pg mLâ1 for AFP.
An ultrasensitive sandwich-type electrochemical immunosensor was developed for quantitative monitoring of Alpha fetoprotein (AFP). To achieve this objective, an incorporated signal amplification strategy of platinum nanoparticles anchored on cobalt oxide/graphene nanosheets (Pt NPs/Co3O4/graphene) was proposed by acting as the label of secondary antibodies. The prepared label not only empowered by advantages of each component but exhibited better electrochemical performance than single Pt NPs, Co3O4 and graphene, which has shown large specific surface area and good catalytic activity towards the reduction of H2O2. Meanwhile, the nanocomposite of gold nanoparticles adhered on 3-mercaptopropyltriethoxysilane functionalized graphene sheets (Au@MPTES-GS) was used as matrix to accelerate electron transfer and immobilize primary antibodies in this system. The signal amplification mechanism of the matrix and the label were explored successfully. Under optimal conditions, the electrochemical immunosensor exhibited a wide linear range from 0.1 pg mLâ1 to 60 ng mLâ1 with a low detection limit of 0.029 pg mLâ1for AFP. The proposed immunosensor may have promising application in the clinical diagnosis of AFP and other tumor markers.
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Journal: Analytica Chimica Acta - Volume 986, 15 September 2017, Pages 138-144