کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5130762 | 1490859 | 2017 | 9 صفحه PDF | دانلود رایگان |
- A photoelectrochemical immunosensor based on ZnO NAs and NBs was fabricated.
- Cosensitized structure of ZnO NAs, CdS and Mn improved the photocurrent response.
- An excellent sensitivity and selectivity of NDPK-A detection was obtained.
Nucleoside diphosphatase kinase A (NDPK-A) is a metastasis-suppressor protein and a biomarker that act on a wide range cancer cells to inhibit the potential metastasis. Herein, we present a simple photoelectrochemical immunosensor based on ZnO nanorod arrays for the sensitive detection of NDPK-A. The ZnO nanorod arrays cosensitized with CdS nanoparticles and Mn2+ displayed a high and stable photocurrent response under irradiation. After anti-NPDK-A nanobodies were immobilized to the ZnO nanorod arrays, the proposed immunosensor can be utilized for detecting NPDK-A by monitoring the changes in the photocurrent signals of the electrode resulting from immunoreaction. Accordingly, the well-designed immunosensor exhibited a low limit of detection (LOD) of 0.3 pg mLâ1 and a wide linear range from 0.5 pg mLâ1 to 10 μg mLâ1. The R2 of the regression curve is 0.99782. Meanwhile, the good stability, reproducibility and specificity of the resulting photoelectrochemical biosensor are demonstrated. In addition, the presented work would offer a novel and simple approach for the detection of immunoreactions and provide new insights in popularizing the diagnosis of NPDK-A.
265
Journal: Analytica Chimica Acta - Volume 973, 22 June 2017, Pages 82-90