کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5131416 | 1490888 | 2016 | 6 صفحه PDF | دانلود رایگان |
- A turn-on fluorescence technique is developed for dopamine detection by using one-step selective reaction between resorcinol and dopamine.
- The limit of detection is 1.8Â nMÂ (S/NÂ =Â 3).
- This detection could be completed within 5Â min.
- The method has been demonstrated to successfully detect dopamine in human urine samples with high recovery ratio of 97.84%-103.50%.
A simple, fast and low-cost method for dopamine (DA) detection based on turn-on fluorescence using resorcinol is developed. The rapid reaction between resorcinol and DA allows the detection to be performed within 5 min, and the reaction product (azamonardine) with high quantum yield generates strong fluorescence signal for sensitive optical detection. The detection exhibits a high sensitivity to DA with a wide linear range of 10 nM-20 μM and the limit of detection is estimated to be 1.8 nM (S/N = 3). This approach has been successfully applied to determine DA concentrations in human urine samples with satisfactory quantitative recovery of 97.84%-103.50%, which shows great potential in clinical diagnosis.
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Journal: Analytica Chimica Acta - Volume 944, 9 November 2016, Pages 51-56