کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5135004 | 1493408 | 2017 | 8 صفحه PDF | دانلود رایگان |
- Universal GC-FID method for analysis of volatile amines.
- Separation of over 30 amines and other basic polar species in a single 16Â min run.
- Method was fully validated for over 25 of the amines and other basic polar species and implemented for pharmaceutical analysis.
- Useful method for analysis of other basic polar species beyond the validated list described in this study.
Volatile amines are among the most frequently used chemicals in organic and pharmaceutical chemistry. Synthetic route optimization often involves the evaluation of several different amines requiring the development and validation of analytical methods for quantitation of residual amine levels. Herein, a simple and fast generic GC-FID method on an Agilent J&W CP-Volamine capillary column (using either He or H2 as the carrier gas) capable of separating over 25 volatile amines and other basic polar species commonly used in pharmaceutical chemistry workflows is described. This 16Â min method is successfully applied to the analysis and quantitation of volatile amines in a variety of pharmaceutically-related drugs and synthetic intermediates. Method validation experiments showed excellent analytical performance in linearity, recovery, repeatability, and limit of quantitation and detection. In addition, diverse examples for the application of this method to the simultaneous determination of other amine-related chemicals in reaction mixtures are illustrated, thereby indicating that these GC-FID method conditions can be effectively used as starting point during method development for the analysis of other basic polar species beyond the validated list of amines described in this study.
Journal: Journal of Chromatography A - Volume 1518, 6 October 2017, Pages 70-77