Microwave-assisted micellar extraction of organic and inorganic iodines using zwitterionic surfactants

- A novel application of MAE using SB3-12 as extractant was presented.
- The proposed method involved the use of UHPLC-Q-TOF/MS.
- The extraction method was efficient and economic for the detection of iodine compounds.
- The method was applied to a determination of kelp samples.

Zwitterionic surfactant, used as extractant in microwave-assisted extraction (MAE) was investigated for the first time to extract organic and inorganic iodines from kelp samples. Optimized conditions for the MAE were 200 W of microwave irradiation power, 100 °C of extraction temperature, 10 min of microwave irradiation time, 1 g of sample, and 20 mL of solvent volume. Ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) was used for the quantitative and qualitative analyses of the iodines. Under the optimum experimental conditions, KI, MIT and DIT were identified in kelp samples, the limits of detection of these analytes were ranged between 3.39 and 6.31 ng/mL. The recoveries for spiked samples obtained from different areas were all higher than 92.48%. Compared with the ultrasound-assisted extraction, the proposed method is faster and more effective. Thus, the combination of zwitterionic surfactant-MAE and UHPLC-Q-TOF/MS made up a simple, rapid and effective approach for extraction and determination of iodine compounds in complex seaweed materials.