کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5135290 1493413 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Purification of dirucotide, a synthetic 17-aminoacid peptide, by ion exchange centrifugal partition chromatography
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Purification of dirucotide, a synthetic 17-aminoacid peptide, by ion exchange centrifugal partition chromatography
چکیده انگلیسی


- Dirucotide is a synthetic peptide developed for the treatment of multiple sclerosis.
- Dirucotide purification was achieved by centrifugal partition chromatography (CPC).
- Ion-exchange CPC mode is an efficient tool in the field of peptide purification.
- Scale up increases the recovery of the purified dirucotide.

Dirucotide is a synthetic drug candidate for the treatment of multiple sclerosis. This 17-aminoacid peptide was successfully purified by ion exchange centrifugal partition chromatography. The optimized conditions involved the biphasic methyl tert-butyl ether/acetonitrile/n-butanol/water (2:1:2:5, v/v) solvent system in the descending mode, the di(2-ethylhexyl)phosphoric acid cation-exchanger with an exchanger (di(2-ethylhexyl)phosphoric acid)/dirucotide mole ratio of 100 and Ca2+ ions in aqueous solution as displacer. Critical impurities were efficiently eliminated and dirucotide was recovered in high yield and purity (69% and 98%, respectively) and with a productivity of 2.29 g per liter of stationary phase per hour.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1513, 1 September 2017, Pages 78-83
نویسندگان
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