کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5135744 1493450 2016 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Optimal mixing rate in linear solvent strength gradient liquid chromatography. Balanced mixing program
ترجمه فارسی عنوان
میزان اختلاط بهینه در روش کروماتوگرافی مایع مغناطیسی خطی خطی. برنامه مخلوط متعادل
کلمات کلیدی
ظرفیت قله، ظرفیت جداسازی، سرعت تجزیه و تحلیل، برگزاری بی نظیر متعادل برنامه مخلوط متعادل، میزان مخلوط کردن، میزان مخلوط کردن ابعاد، نرخ مخلوط بهینه،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The mixing rate (Rϕ) is the temporal rate of increase in the solvent strength in gradient LC. The optimal Rϕ (Rϕ,Opt) is the one at which a required peak capacity of gradient LC analysis is obtained in the shortest time. The balanced mixing program is a one where, for better separation of early eluting solutes, the mixing ramp is preceded by a balanced isocratic hold of the duration depending on Rϕ. The improvement in the separation of the earlier eluites due to the balanced programming has been evaluated. The value of Rϕ,Opt depends on the solvent composition range covered by the mixing ramp and on the column pressure conditions. The Rϕ,Opt for a column operating at maximum instrumental pressure is different from Rϕ,Opt for a column operating below the instrumental pressure limit. On the other hand, it has been shown that the difference in the Rϕ,Opt values under different conditions is not very large so that a single default Rϕ previously recommended for gradient analyses without the isocratic hold also yields a good approximation to the shortest analysis time for all conditions in the balanced analyses. With or without the initial balance isocratic hold, the recommended default Rϕ is about 5%/t0 (5% increase in the solvent strength per each t0-long increment in time) for small-molecule samples, and about an order of magnitude slower (0.5%/t0) for protein samples. A discussion illustrating the use of the optimization criteria employed here for the techniques other than LSS gradient LC is included.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1476, 9 December 2016, Pages 35-45
نویسندگان
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