کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5136646 | 1494020 | 2016 | 34 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Saponins from Panax bipinnatifidus Seem.: New strategy of extraction, isolation, and evaluation of tyrosinase inhibitory activity based on mathematical calculations
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A hyphenated accelerated solvent extraction (ASE) technique coupled with centrifugal partition chromatography (CPC), ultra high performance liquid chromatography (UPLC), and mass spectrometry (MS) was established. The CPC fractions were prepared using the hyphenated technique. Subsequently, tyrosinase inhibitory activities of the CPC fractions were experimentally evaluated, and the activities of individual components were calculated theoretically. This new approach was applied to saponin fractions obtained from 10.0Â g of raw Panax bipinnatifidus Seem. via a biphasic solvent system of ethyl acetate/n-butanol/methanol/water (2:3:2:5, v/v/v/v). The CPC fractions monitoring was performed using an online UPLC/PDA system at 5-min intervals. The tyrosinase inhibitory activities of all fractions were analysed using the fluorescence method. Mathematical calculations indicated that the inhibition rates of the ginsenosides Rh1, Rh2, Rg1, Rg2, and chikusetsusaponin L5 were all above 50.00%, showing potential for further development. The results were confirmed by comparison with authentic standards.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1039, 15 December 2016, Pages 79-87
Journal: Journal of Chromatography B - Volume 1039, 15 December 2016, Pages 79-87
نویسندگان
Yuchi Zhang, Chong Shi, Chunming Liu, Min Yu, Yanjuan Qi, Sainan Li,