کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5137812 1494590 2017 16 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantification of paracetamol and 5-oxoproline in serum by capillary electrophoresis: Implication for clinical toxicology
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Quantification of paracetamol and 5-oxoproline in serum by capillary electrophoresis: Implication for clinical toxicology
چکیده انگلیسی
High anion gap metabolic acidosis frequently complicates acute paracetamol overdose and is generally attributed to lactic acidosis or compromised hepatic function. However, metabolic acidosis can also be caused by organic acid 5-oxoproline (pyroglutamic acid). Paracetamol's toxic intermediate, N-acetyl-p-benzoquinoneimine irreversibly binds to glutathione and its depletion leads to subsequent disruption of the gamma glutamyl cycle and an excessive 5-oxoproline generation. This is undoubtedly an underdiagnosed condition because measurement of serum 5-oxoproline level is not readily available. A simple, cost effective, and fast capillary electrophoresis method with diode array detection (DAD) for simultaneous measurement of both paracetamol (acetaminophen) and 5-oxoproline in serum was developed and validated. This method is highly suitable for clinical toxicology laboratory diagnostic, allowing rapid quantification of acidosis inducing organic acid 5-oxoproline present in cases of paracetamol overdose. The calibration dependence of the method was proved to be linear in the range of 1.3-250 μg mL−1, with adequate accuracy (96.4-107.8%) and precision (12.3%). LOQ equaled 1.3 μg mL−1 for paracetamol and 4.9 μg mL−1 for 5-oxoproline.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 145, 25 October 2017, Pages 616-620
نویسندگان
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