کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5138241 | 1494599 | 2017 | 26 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evaluation of size-exclusion chromatography for the analysis of phosphorothioate oligonucleotides
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Evaluation of size-exclusion chromatography for the analysis of phosphorothioate oligonucleotides Evaluation of size-exclusion chromatography for the analysis of phosphorothioate oligonucleotides](/preview/png/5138241.png)
چکیده انگلیسی
We evaluated size exclusion chromatography (SEC) for the detection of high-order structure of phosphorothioate oligonucleotides (PS-oligo). Because of strong interaction between PS-oligo and column packing material, peaks were broader and elution time was longer than those of the corresponding natural DNA oligonucleotides. However, single- and double-stranded structures of PS-oligo were clearly separated and discriminated, while single-stranded with high-order structures such as G-quadruplex and hairpin structure were not distinguished from each other.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 136, 20 March 2017, Pages 55-65
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 136, 20 March 2017, Pages 55-65
نویسندگان
Atsuko Shimoyama, Aki Fujisaka, Satoshi Obika,